Analysis of animal model of atopic dermatitis based on characteristics of clinical symptoms traditional Chinese and Western medicine / 中国中药杂志

Based on the clinical characteristics of atopic dermatitis( AD) in traditional Chinese medicine( TCM) and Western medicine,the existing animal models were analyzed,and the coincidence degree,advantages and disadvantages between the models and the clinical manifestations of AD were evaluated,so as to provide reference for establishing a rational animal model. After consulting relevant literatures in recent years and summarizing the existing modeling methods,it is found that spontaneous,transgenic/gene knockout models were highly consistent,but with high breeding conditions and expensive prices. The hapten-induced model was low in cost and fast in modeling. It revealed the corresponding mechanism of AD to a certain extent,but did not fully reflect the state of the entire process of AD. The modeling method was guided by Western medicine,but with a lack of pathogenic factors of traditional Chinese medicine,and so has certain limitations in TCM research. Therefore,it is necessary to combine the etiology,pathogenesis and clinical mani-festations of AD with traditional Chinese and Western medicine,so as to improve the coincidence degree between the model and the characteristics of clinical symptoms and lay the foundation for in-depth studies on AD.

Analysis of filaggrin 2 gene polymorphisms in patients with atopic dermatitis

Abstract Background: Polymorphisms of the filaggrin 2 gene (rs 12568784 and rs 16899374) are associated with persistent atopic dermatitis in African American patients. Filaggrin 2 is a protein with a function similar to filaggrin and also encoded in the epidermal differentiation complex on chromosome 1q21. Objective: To evaluate the polymorphisms in the filaggrin 2 gene (rs 12568784 and rs 16899374) in children and adults with atopic dermatitis and to verify the association of these with the severity of the clinical picture, presence of other allergic diseases, and socio-demographic factors. Method: The study was carried out with patients and control group. Questionnaires were used to evaluate ethnicity, sex, age, family history, scoring, atopic dermatitis (SCORAD), among other parameters. Genotyping of the filaggrin 2 gene was performed by real-time polymerase chain reaction. Results: Forty-eight patients and 83 controls were evaluated. No correlation was found between the variables studied in patients with atopic dermatitis and polymorphisms, no significant difference between the prevalence of polymorphisms in the patients and in the control group p > 0.05. Study limits: The exclusive use of self-reported ethnicity information and the sample size. Results: The results of this work can be an incentive for the study of the polymorphisms in atopic dermaititis, considering the characteristic of the Brazilian multi ethnic population. Conclusion: This is an unpublished work in Brazil and the first study in the world to have a control group to evaluate alterations in the gene of filaggrin 2.

Particular characteristics of atopic eczema in tropical environments. The Tropical Environment Control for Chronic Eczema and Molecular Assessment (TECCEMA) cohort study

Abstract: Background: Atopic dermatitis is a prevalent health problem in the world. Allergic sensitization is an important risk factor, but the roles of other factors, inherent in tropic region, are unknown. Objective: A cohort study was designed in a tropical city to investigate molecular and environmental risk factors for eczema, considering as particular features perennial exposure to mites, poor living conditions and others tropical characteristics. Methods: 433 patients were included at baseline and biological samples were collected during 24 months of follow-up. Clinical information was collected using questionnaires (SCORAD, DLQI and a subjective scale) during each clinical assessment. Results: The prevalence of atopic eczema was 93%, with similar frequency between children and adults; parents history of eczema and polysensitization to mites, dogs, cats, cockroaches and birds, were risk factors for severe and persistent eczema and allergic comorbidities. Food sensitization was present in 16% of patients but food-induced allergies were scarce. Psychiatric, dental and ocular disorders were the most frequent non-allergic comorbidities. Study limitations: selection bias. Conclusion: We presented a tropical cohort of patients with eczema and we identified some risk factors for severe and persistent dermatitis. Some patterns of sensitization were associated with severe eczema and respiratory symptoms, and the natural history of "atopic march" is different to that described in some industrialized countries. The collection of biological samples will contribute to the understanding of the gene/environment interactions leading to allergy inception and evolution.

An Analysis of the Filaggrin Gene Polymorphism in Korean Atopic Dermatitis Patients

Research of the FLG mutation in various ethnic groups revealed non-overlapping mutation patterns. In addition, Japanese and Chinese atopic patients showed somewhat different mutations. These ethnic differences make the research on Korean patients mandatory; however, no systematic research on Korean atopic dermatitis (AD) patients has been performed. This study aims to investigate the genetic polymorphism of FLG in Korean atopic dermatitis patients. The study was made up of three groups including 9 Ichthyosis vulgaris (IV) patients, 50 AD patients and 55 normal controls: the ichthyosis group was incorporated due to the reported association between the FLG mutation and IV. In comparison to other sequencing methods, the overlapping long-range PCR was used. We revealed the genetic polymorphism of filaggrin in Koreans, and at the same time, we discovered nonsense mutations in p.Y1767X and p.K4022X in Korean AD patients. By using FLG sequencing techniques confirmed in this study, new mutations or genetic polymorphisms with ethnic characteristics would be detected and further larger studies of repeat number polymorphisms could be performed.

Dermatopathia pigmentosa reticularis and atopic dermatitis: a case report of two siblings

Dermatopathia pigmentosa reticularis is a rare reticulate pigmentary disorder which starts during infancy or childhood and consists of a triad of generalized reticulate hyperpigmentation, nonscarring alopecia, and nail dystrophy. We report 2 sisters with this syndrome with an additional association of atopic dermatitis in the elder one

FCepsilonRI Gene Promoter Polymorphisms and Total IgE Levels in Susceptibility to Atopic Dermatitis in Korea

IgE-dependent activation of mast cells and basophils through the high-affinity IgE receptor (FcepsilonRI) is involved in the pathogenesis of allergen-induced immune responsiveness in atopic diseases like atopic dermatitis (AD). We sought to determine FcepsilonRI gene polymorphisms are associated with AD in Korean patients, and analyzed the relevance of FcepsilonRI gene polymorphisms and serum IgE levels. We conducted a case-control association analysis (175 patients and 56 controls) of Korean subjects. Genotyping was performed using the TaqMan fluorogenic 5' nuclease assay, and serum levels of IgE were measured using a fluorescence enzyme immunoassay. We found that there were no significant relationships between FcepsilonRI and AD, although there were trends towards an association between the 66T>C (rs2251746) polymorphism and total serum IgE levels in the Korean AD patients. In conclusion, while the 66T>C (rs2251746) of the FcepsilonRIalpha polymorphism may be linked to AD and higher serum IgE levels, polymorphisms in the FcepsilonRIbeta gene did not confer susceptibility to AD in our patient sample.

Anasarca e hipoalbuminemia associadas à dermatite atópica: relato de caso / Generalized edema and hypoalbuminemia associated to atopic dermatitis: case report

Objetivo: Relatar um caso de criança com Dermatite Atópica grave, que evoluiu com perda protéica e diminuição dos níveis de albumina sérica durante episódio de agudização das lesões cutâneas. Descrição do caso: Paciente de dois anos e dez meses foi encaminhado ao ambulatório de Alergia e Imunologia do Instituto da Criança - HCFMUSP com diagnóstico de Dermatite Atópica desde 18 meses de vida. Em episódio de infecção bacteriana secundária, evoluiu com edema generalizado, hipoproteinemia e hipoalbuminemia decorrente da perda cutânea de albumina sérica. Após terapêutica com antibióticos, corticoterapia tópica e sistêmica e infusão de albumina, houve melhora clínica com controle do processo inflamatório e elevação dos níveis sé ricos de albumina. comentários: Embora a hipoproteinemia secundária ao processo inflamatório da Dermatite Atópica seja uma ocorrência rara, nos casos graves deve ser realizada a avaliação dos níveis de albumina para detecção precoce e tratamento desta complicação.

Dermatitis atopica / Atopic dermatitis

La dermatitis atópica es una enfermedad inflamatoria crónica de la piel que se caracteriza por episodios recurrentes de eczema, xerosis y un intenso prurito. Con frecuencia en los pacientes se asocia con alergia respiratoria y con una historia familiar de atopia. Dependiendo de la edad del paciente se reconocen distintas fases en las cuales las manifestaciones clínicas son diferentes. La prevalencia de la dermatitis atópica se ha incrementado en los últimos años, constituyéndose como una de las causas dermatológicas más importantes de consulta

Corticosteroides y el síndrome eccema atópico y/o dermatitis / Corticosteroids and atopic eczema/dermatitis syndrome

La dermatitis atópica es una de las afecciones de la piel más frecuentes. Caracterizada por una constelación de signos y síntomas, presentan un cuadro fisiopatológico complejo en donde están involucrados numerosos mecanismos inmunológicos, enzimáticos e inespecíficos. Los corticosteroides actúan como inmunomoduladores a diferentes niveles. La elección del corticosteroide tópico adecuado debe recaer sobre aquel que ofrece las mayores ventajas para lograr una remisión rápida y sostenida de los síntomas. El uso posterior intermitente permite su administración sin generar efectos colaterales. Aún cuando existen otras alternativas inmunomoduladoras, los corticosteroides tópicos siguen siendo la medicación de primera elección en el tratamiento de la dermatitis atópica

Perfil de risco entre os pacientes com história de atopia quanto às dermatites alérgicas de contato / Risk profile among patients with a history of atopy for allergic contact dermatitis

Verificar a freqüência da associaçäo entre a presença de histórico pessoal e/ou familiar de atopia e a positividade dos testes epicutâneos para avaliaçäo de dermatite alérgica de contato; Verificar as especifidades quanto aos tipos de substâncias implicadas, à faixa etária e ao grau de intensidade das reaçöes aos testes. Foram avaliados 136 pacientes que consultaram no Setor de Alergia Cutânea do Serviço e realizaram os testes epicutâneos entre 1998 e 2000. Utilizou-se uma bateria padräo com 30 substâncias, com retirada dos testes em 48 horas e leitura final em 96 horas. Realizou-se aferiçäo dos resultados de acordo com as normas internacionais.Näo houve diferença estatisticamente significativa entre os grupos com e sem história de atopia quanto à freqüência de positividade aos testes epicutâneos. O grau de intensidade das reaçöes foi maior no grupo com história de atopia (OR: 1,36). O grupo com história de atopia teve uma média de idade de 39,8 anos e o grupo sem a história uma média de 45,9 anos. Entre os atópicos, verificou-se o sulfato de níquel com significância estatística como a principal substância (p<0,05). Näo há diferenças da freqüência em geral quanto à reatividade aos testes epicutâneos. Os pacientes com história de atopia pessoal e/ou familiar provavelmente constituem um grupo de risco para o desenvolvimento de dermatites alérgicas de contato mais intensas e säo, particularmente, reativos ao sulfato de níquel.

Atopic dermatitis: on the role of epidermal barrier function, cytokine pattern and leukemia inhibitory factor

A dermatite atópica é um dos distúrbios inflamatórioscrônicos mais comuns em dermatologia com uma incidência cada vez maior nos países industrializados. Sua etiologia ainda näo foi definida, mas nosso conhecimento sobre a genética, imunologia e outros fatores da dermatite atópica têm aumentado consideravelmente. Säo discutidos neste artigo o principal papel da interaçäo entre o fator inibitório da leucemia e a interleucina 4. Esta revisäo fornece uma hipótese provocante sobre a natureza desta doença: dermatite atópica - a displasia ectodérmica mais comum?

Etiopatogenia da dermatite atópica: revisäo bibliográfica dos últimos10 anos / Etiopathogenesis of atopic dermatitis: review of the last ten years

Nesta revisäo da etiologia e patogênese da dermatite atópica, os autores estudaram os vários aspectos desta manifestaçäo complexa e multifatorial em especial predisposiçäo genética e anormalidades imunológicas centralizadas em uma disfunçäo dos T-linfócitos helper e supressor, produçäo de níveis altos de IgE, imunidade celular deprimida e liberaçäo de mediadores químicos de mastócitos e basófilos com a ativaçäo de células inflamatórias e interleucinas, resultando em inflamaçäo crônica local e em hiperreatividade cutânea observada nestes pacientes

Etiopatogenia da dermatite atópica: revisäo bibliográfica dos últimos 10 anos / Etiopathogenesis of atopic dermatitis: review of the last 10 years

Nesta revisäo da etiologia e patogênese da Dermatite Atópica, os autores estudaram os vários aspectos desta manifestaçäo complexa e multifatorial, em especial predisposiçäo genética e anormalidades imunológicas centralizadas em uma disfunçäo dos T-linfócitos helper e supressor, produçäo de níveis altos de IgE, imunidade celular deprimida e liberaçäo de mediadores químicos de mastócitos e basófilos, com a ativaçäo de células inflamatórias e interleucinas, resultado em inflamaçäo crônica local e em hiperreatividade cutânea observada nestes pacientes

Disidrose: estudo prospectivo de 600 casos / Dyshidrosis: prospective study of 600 cases

O autor realizou um estudo prospectivo em 600 pacientes portadores de disdrose. Após uma revisäo bibliográfica a respeito mostra os resultados obtidos em relaçäo ao sexo, raça, idade, profissäo, localizaçöes das lesöes, meses de freqüência e possíveis causas etiológicas

Dermatosis atópica en el niño: etiopatogenia, tratamento y evolución / Atopic dermatitis in child: etiopathogeny treatment and evolution

Se realizó un estudio de los últimos adelantos sobre las alteracions inmunitatrias en la dermatosis atópica. El estudio abarcó 80 pacientes tratados en el Hospital Pediátrico de la ciudad de Santa Clara, Cuba. Se determinaron la patogénesis, el cuadro clínico, los exámenes de laboratorio, el tratamiento y la evolución. Entre los resultados se destaca predominio de la aparición del primer brote antes del año de edad. Los antecedentes patológicos personales y familiares de atopia fueron informados en frecuencia elevada. Se valoraron los tratamientos habitualmente utilizados y se les administró extracto placentario en vacuna a los casos de evolución no satisfactoria